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Progenitor (CFC) Assays FAQs
How well can your in vitro assays predict clinical compound-induced hematotoxicity/myelosuppression?
What kinds of compounds can be tested in your predictive hematotoxicity assays?
Why do you use functional progenitor cell (CFC) assays instead of just a proliferation assay?
Has the Colony-Forming Cell (CFC) assay been validated as a means to predict compound-induced hematotoxicity?
How much compound do you need to test and what format should we provide it to you in?
We are happy to accept the compound in powder format or at a stock concentration 1000x higher than the highest desired test concentration. Stock solutions may be made up in DMSO or another solvent of your choice.
How fast is your turn-around time once we provide you with our compounds?
Final reports are generally available 2 weeks following study completion.
How many compounds can you test at once?
How many concentrations do you suggest we test?
What cell types do you use in your CFC assays?
We can also test bone marrow and peripheral blood from diseased patient samples.
We also routinely test bone marrow MNCs from mouse, rat, canine and NHP.
What species can you test our compound(s) on?
If you require testing of cells from other species, please CONTACT US to discuss your needs. Please be aware that some of the cytokines required to support the various progenitors in CFC assays can be exquisitely species-specific, so not all species may be able to be accommodated.
Can you perform molecular analyses on cells derived from the colonies after the CFC assay has been done?
Depending on the application, molecular analyses can be performed on cells from individual colonies or on cells from pooled colonies.
Flow Cytometry Services FAQs
Can you perform multi-color flow analysis? If so, how many colors?
For studies requiring a very large number of parameters to be assessed at the same time, we would generally employ mass cytometry, utilizing a DVS CyTOF analyzer. This platform allows for up to 30 different parameters to be tested on a single cell and requires SPADE analysis.
Can you analyze flow data for us?
For very high parameter mass cytometry experiments, we typically perform SPADE analysis using the CytoBank platform.