ColonyGEL™ Media for CFC Assays

High Performance Methylcellulose-Based Media for Multi-Species Colony-Forming Cell Assays

colonygel

About Colony Forming Cell Assays

Hematopoietic progenitors with lineage-restricted (less primitive) or multi-lineage (more primitive) potential form morphologically distinct colonies when grown in a suitable semi-solid medium containing appropriate growth factors and other supplements. To form the specific colony type that characterizes its potential, a progenitor cell must both proliferate and differentiate within the supportive matrix. Colony-forming cell (CFC) assays therefore have great utility in the identification and enumeration of the different types of functional progenitors in hematopoietic tissues (e.g., bone marrow, cord blood, fetal liver) and manipulated cell populations (e.g., purified, expanded, frozen/thawed, drug treated, etc.). For a variety of reasons, methylcellulose has become the matrix of choice for semi-solid media for CFC assays. Pre-screening and qualification of the various components of methylcellulose-based media allow for media products that are highly reproducible in their ability to support vigorous and robust growth of the various types of progenitors. ColonyGEL™ meets the highest standards of methylcellulose-based media for hematopoietic colony-forming cell assays.

About ColonyGEL™

ReachBio Research Labs knows colony assays! ReachBio Research Labs has been performing hematopoietic progenitor/colony-forming cell (CFC) assays and other primary cell-based assays under contract for premier biotechnology and pharmaceutical companies since 2007. Our scientists have decades of prior experience performing CFC assays in clinical, industrial and academic settings. Our Chief Scientific Officer, Dr. Emer Clarke, has trained countless individuals around the world on hematopoietic and mesenchymal CFC assays. She also designed and managed the first global proficiency testing program for standardizing hematopoietic CFC assays. Since our inception, we have used our ColonyGEL™ media for our contract services for such high value industrial applications as drug screening, hemotoxicity testing, cancer stem cell assays, cytokine mimetic identification, evaluation of expanded cell populations, monitoring mobilization kinetics, etc. We are now making our high quality methylcellulose-based ColonyGEL™ media widely available to the broader research community. We guarantee that its performance will meet or exceed any media of its kind on the market today! Optimized ColonyGEL™ formulations are available for a variety of species.

rat mouse monkey human dog

Features and Benefits of ColonyGEL™

  • Pre-screened and carefully selected components

  • Reproducible and reliable – used in ReachBio Research Lab’s own contract service labs for high value drug development applications

  • Great optical clarity

  • Manufactured in the USA in accordance with the highest standards

  • Meets or beats the performance of the current “gold standard” CFC assay media

  • Various optimized formulations available

    –   with or without Epo

    –   standard or enhanced cytokine content

    –   species-specific formulations

    –   base medium also available – allows addition of your own growth

        factors for unique applications

  • Suitable for identifying and enumerating:

    –   CFU-E

    –   BFU-E

    –   CFU-G

    –   CFU-M

    –   CFU-GM

    –   CFU-GEMM

    –   Pre-B (mouse only)

visit www.colonygel.com for more information

PRODUCTS

ColonyGEL™ for HUMAN Cells

human

Catalogue Number Product Name Cytokines Colony Types Supported Size Product Info Sheet Price (USD)
1100
ColonyGEL 1100
Stock Methylcellulose Medium
none n/a 100 mL download

$115.00 Add to cart

1101
ColonyGEL 1101
Human Base Medium
none n/a 90 mL download

$175.00 Add to cart

1102
ColonyGEL 1102
Human Complete Medium
rhSCF;  rhGM-CSF; rhIL-3; rhEPO CFU-E; BFU-E; CFU-G; CFU-M; CFU-GM; CFU-GEMM 100 mL download

$330.00 Add to cart

1103
ColonyGEL 1103
Human Complete Medium Without EPO
rhSCF; rhGM-SCF; rhIL-3 CFU-G; CFU-M; CFU-GM 100 mL download

$300.00 Add to cart

1104
ColonyGEL 1104
Human Enriched Complete Medium
rhSCF; rhGM-CSF;rhG-CSF; rhIL-3; rhIL-6; rhEPO CFU-E; BFU-E; CFU-G; CFU-M; CFU-GM; CFU-GEMM 100 mL download

$420.00 Add to cart

1150
ColonyGEL 1150
Human Medium for Multiple Myeloma CFC
PHA-LCM MM-CFC 100 mL download

$370.00 Add to cart

ColonyGEL™ for MOUSE Cells

mouse

Catalogue Number Product Name Cytokines Colony Types Supported Size Product Info Sheet Price (USD)
1100
ColonyGEL 1100
Stock Methylcellulose Medium
none n/a 100 mL download

$115.00 Add to cart

1201
ColonyGEL 1201
Mouse Base Medium
none n/a 90 mL download

$225.00 Add to cart

1202
ColonyGEL 1202
Mouse Complete Medium
rmSCF; rmIL-3; rmIL-6; rhEPO CFU-E; BFU-E; CFU-G; CFU-M; CFU-GM; CFU-GEMM 100 mL download

$385.00 Add to cart

1203
ColonyGEL 1203
Mouse Complete Medium Without EPO
rmSCF; rmIL-3; rmIL-6 CFU-G; CFU-M; CFU-GM 100 mL download

$340.00 Add to cart

1205
ColonyGEL 1205
Mouse Pre-B Cell Medium
rmIL-7 CFU pre-B 100 mL download

$315.00 Add to cart

ColonyGEL™ for RAT Cells

rat

Catalogue Number Product Name Cytokines Colony Types Supported Size Product Info Sheet Price (USD)
1100
ColonyGEL 1100
Stock Methylcellulose Medium
none n/a 100 mL download

$115.00 Add to cart

1301
ColonyGEL 1301
Rat Base Medium
none n/a 90 mL download

$260.00 Add to cart

1303
ColonyGEL 1303
Rat Complete Medium Without EPO
rmSCF; rrGM-CSF; rrIL-3 CFU-G; CFU-M; CFU-GM 100 mL download

$400.00 Add to cart

ColonyGEL™ for NHP Cells

monkey

Catalogue Number Product Name Cytokines Colony Types Supported Size Product Info Sheet Price (USD)
1100
ColonyGEL 1100
Stock Methylcellulose Medium
none n/a 100 mL download

$115.00 Add to cart

1401
ColonyGEL 1401
NHP Base Medium
none n/a 90 mL download

$215.00 Add to cart

1402
ColonyGEL 1402
NHP Complete Medium
rhSCF; rhGM-CSF; rhIL-3; rhEPO CFU-E; BFU-E; CFU-G; CFU-M; CFU-GM; CFU-GEMM 100 mL download

$385.00 Add to cart

1403
ColonyGEL 1403
NHP Complete Medium Without EPO
rhSCF; rhGM-CSF; rhIL-3;  CFU-G; CFU-M; CFU-GM; 100 mL download

$350.00 Add to cart

ColonyGEL™ for CANINE Cells

dog

Catalogue Number Product Name Cytokines Colony Types Supported Size Product Info Sheet Price (USD)
1100
ColonyGEL 1100
Stock Methylcellulose Medium
none n/a 100 mL download

$115.00 Add to cart

1501
ColonyGEL 1501
Canine Base Medium
none n/a 90 mL download

$240.00 Add to cart

1503
ColonyGEL 1503
Canine Complete Medium Without EPO
proprietary CFU-G; CFU-M; CFU-GM 100 mL download

$440.00 Add to cart

ColonyGEL™ Support Products

Catalogue Number Product Name Unit Size Product Info Sheet Price (USD)
3011 Blunt End Needles for CFC Assays 25 needles per package download

$35.00 Add to cart

Did You Know?

ReachBio Research Labs provides a number of REACHBIO QUALIFIED™ primary human cell products for use in CFC assays!

Each batch of our REACHBIO QUALIFIED™ cell products are functionally pre-qualified in CFC assays using our  ColonyGEL methylcellulose-based media. The frequency of myeloid and erythroid progenitors, as well as a recommended plating concentration for the CFC assay are provided for each batch of REACHBIO QUALIFIED™ cells.

The following REACHBIO QUALIFIED™ cell products are available: